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KMID : 0359819950240020115
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Journal of Korean Neurosurgical Society
1995 Volume.24 No. 2 p.115 ~ p.122
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A Study on the Role of Protein Kinase C upon the Acetylcholine Release in the Rat Hippocampus
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Abstract
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The effects and interactions of 4β-phorbol 12. 13-dibutyrate (PDB) and polymyxin B (PMB) with adenosine on the electrically-evoked acetylcholine (ACh) release were studied in rate hippocampus. Slices from rat hippocampus were equilibrated with
3H-choline and the release of the labelled product. 3H-ACh. which was evoked by electrical stimulation (3Hz. 2ms. 5VCm1. rectangular pulses) was measured.
PDB (0.3-10μM). a selective protein kinase C (PKC) activator. increased the evoked ACh release in a dose related fashion with an increase in the basal rate of release. The effects of 1μM PDB were significantly inhibited by 0.3μM tetrodotoxin
(TTX)
pretreatment or Ca++-free medium. PMB (0.03-1mg). a selective PKC inhibitor. decreased the ACh release in a dose dependent manner with an increase in the basal rate of release.
Adenosine (1-01μM) decreased the ACh release without changing the basal rate of release. and this effect was significantly inhibited by 8-cyclopentyl-1.3-dipropylxanthine (2μM). a selective A1-receptor antagonist treatment. However. adenosine
effects
were not affected by PDB and PMB.
These results indicate that the PKC play a role in the ACh release in the rat hippocampus but is not involved in the post-receptor mechanism of the A1-adenosine receptor.
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